Paper THE RABIT: A RAPID AUTOMATED BIODOSIMETRY TOOL FOR RADIOLOGICAL TRIAGE
نویسندگان
چکیده
In response to the recognized need for high throughput biodosimetry methods for use after large-scale radiological events, a logical approach is complete automation of standard biodosimetric assays that are currently performed manually. The authors describe progress to date on the RABIT (Rapid Automated BIodosimetry Tool), designed to score micronuclei or -H2AX fluorescence in lymphocytes derived from a single drop of blood from a fingerstick. The RABIT system is designed to be completely automated, from the input of the capillary blood sample into the machine to the output of a dose estimate. Improvements in throughput are achieved through use of a single drop of blood, optimization of the biological protocols for in situ analysis in multi-well plates, implementation of robotic-plate and liquid handling, and new developments in high-speed imaging. Automating well-established bioassays represents a promising approach to high-throughput radiation biodosimetry, both because high throughputs can be achieved, but also because the time to deployment is potentially much shorter than for a new biological assay. Here the authors describe the development of each of the individual modules of the RABIT system and show preliminary data from key modules. System integration is ongoing, followed by calibration and validation. Health Phys. 98(2):209–217; 2010
منابع مشابه
Development of a Robotically-based Automated Biodosimetry Tool for High-throughput Radiological Triage
To provide the best opportunities for life-saving interventions in the event of a radiological or nuclear threat, there is an urgent need to improve the speed and efficiency of biodosimetric assays for triage and therapy. A rapid automated biodosimetric assay tool used to assess thousands of individual radiation exposure doses is helpful to curb mass panic, and to conserve limited medical resou...
متن کاملDevelopment of a robotically-based automated biodosimetry tool for high-throughput radiological triage
To provide the best opportunities for life-saving interventions in the event of a radiological or nuclear threat, there is an urgent need to improve the speed and efficiency of biodosimetric assays for triage and therapy. A rapid automated biodosimetric system used to assess thousands of individual radiation exposure doses is helpful to curb mass panic, and to conserve limited medical resources...
متن کاملThe RABiT: a rapid automated biodosimetry tool for radiological triage. II. Technological developments.
PURPOSE Over the past five years the Center for Minimally Invasive Radiation Biodosimetry at Columbia University has developed the Rapid Automated Biodosimetry Tool (RABiT), a completely automated, ultra-high throughput biodosimetry workstation. This paper describes recent upgrades and reliability testing of the RABiT. MATERIALS AND METHODS The RABiT analyses fingerstick-derived blood samples...
متن کاملThe RABIT: a rapid automated biodosimetry tool for radiological triage.
In response to the recognized need for high throughput biodosimetry methods for use after large-scale radiological events, a logical approach is complete automation of standard biodosimetric assays that are currently performed manually. The authors describe progress to date on the RABIT (Rapid Automated BIodosimetry Tool), designed to score micronuclei or gamma-H2AX fluorescence in lymphocytes ...
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∗ Post-doctor Research Scientist, Phone: (212) 854-7693, Fax: (212) 854-3304, Email: [email protected]. ABSTRACT This paper presents design, hardware, software, and parameter optimization for a novel robotic automation system. RABiT is a Rapid Automated Biodosimetry Tool for high throughput radiological triage. The design considerations guiding the hardware and software architecture are prese...
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